Development of a comprehensive salivary steroid profile utilising liquid chromatography- tandem mass spectrometry and gas chromatography-mass spectrometry.
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By Mariyam and Dr Angela Taylor- University of Birmingham.
Steroids are important in many biological processes. Most techniques for the profiling of steroids in biofluids rely on coupled mass spectrometry techniques which tend to focus on matrices such as blood (serum/plasma) or urine. Due to the unique enzymatic environment within saliva, steroid composition has been shown to be altered from that in other biofluids (1), therefore prior to quantification, an assessment of the composition of the salivary steroid profile is warranted. Here we used both GC-MS and LC-MS/MS steroid profiling techniques to determine the composition of salivary steroid profiles through the analysis of samples from healthy participants. Using these techniques, both free and conjugated steroids were assessed. A total of 66 steroids were screened with 31 being deemed to be within a quantifiable concentration range and an additional 8 being deemed to be present but bellow the limit of quantification, detectable.
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